Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation - BIG - Biologie à Grande Échelle (BGE)
Article Dans Une Revue Science Année : 2017

Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation

Christophe Bosc
Leticia Peris
Neri Amara
  • Fonction : Auteur
Marie-Jo Moutin

Résumé

Reversible detyrosination of α-tubulin is crucial to microtubule dynamics and functions, and defects have been implicated in cancer, brain disorganization, and cardiomyopathies. The identity of the tubulin tyrosine carboxypeptidase (TCP) responsible for detyrosination has remained unclear. We used chemical proteomics with a potent irreversible inhibitor to show that the major brain TCP is a complex of vasohibin-1 (VASH1) with the small vasohibin binding protein (SVBP). VASH1 and its homolog VASH2, when complexed with SVBP, exhibited robust and specific Tyr/Phe carboxypeptidase activity on microtubules. Knockdown of vasohibins or SVBP and/or inhibitor addition in cultured neurons reduced detyrosinated α-tubulin levels and caused severe differentiation defects. Furthermore, knockdown of vasohibins disrupted neuronal migration in developing mouse neocortex. Thus, vasohibin/SVBP complexes represent long-sought TCP enzymes.

Domaines

Neurobiologie
Fichier principal
Vignette du fichier
Aillaud et al Science_main and suppl data_accepted ms.pdf (13.74 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02345845 , version 1 (04-11-2022)

Identifiants

Citer

Chrystelle Aillaud, Christophe Bosc, Leticia Peris, Anouk Bosson, Pierre Heemeryck, et al.. Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation. Science, 2017, 358 (6369), pp.1448-1453. ⟨10.1126/science.aao4165⟩. ⟨hal-02345845⟩
157 Consultations
78 Téléchargements

Altmetric

Partager

More