%0 Journal Article %T A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment %+ Laboratoire de Radiobiologie des expositions médicales (IRSN/PSE-SANTE/SERAMED/LRMed) %+ Sorbonne Université (SU) %+ Stabilité génétique, cellules souches et radiations (SGCSR (U_1274 / UMR_E_008)) %+ Institut de Biologie François JACOB (JACOB) %+ Université de Lille %+ Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF) %A Ladaigue, Ségolène %A Lefranc, Anne-Charlotte %A Balde, Khadidiatou %A Quitoco, Monica %A Bacquer, Emilie %A Busso, Didier %A Piton, Guillaume %A Dépagne, Jordane %A Déchamps, Nathalie %A Yamakawa, Nao %A Debusschere, Louise %A Han, Chunxue %A Allain, Fabrice %A Buard, Valerie %A Tarlet, Georges %A Paget, Vincent %A Francois, Agnes %A Milliat, Fabien %A Guipaud, Olivier %Z EDF (Groupe Gestion Projet Radioprotection)IRSN %< avec comité de lecture %J iScience %I Elsevier %V 25 %N 12 %P 105482 %8 2022-12-22 %D 2022 %R 10.1016/j.isci.2022.105482 %Z Life Sciences [q-bio]Journal articles %X Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions. Trimmed by the class I α-mannosidases, these structures are quite rare in normal conditions. Here, we show that the expression of the endothelial α-mannosidase MAN1C1 protein decreases after irradiation. We modeled two crucial steps in monocyte recruitment by developing in vitro real-time imaging models. Inhibition of MAN1C1 expression by siRNA gene silencing increases the abundance of high-mannose N-glycans, improves the adhesion of monocytes on endothelial cells in flow conditions and, in contrast, decreases radiation-induced transendothelial migration of monocytes. Consistently, overexpression of MAN1C1 in endothelial cells using lentiviral vectors decreases the abundance of high-mannose N-glycans and monocyte adhesion and enhances transendothelial migration of monocytes. Hence, we propose a role for endothelial MAN1C1 in the recruitment of monocytes, particularly in the adhesion step to the endothelium. %G English %Z Plateforme PAGes (US 41 - UAR 2014 - PLBS), université de Lille %2 https://irsn.hal.science/irsn-03888401/document %2 https://irsn.hal.science/irsn-03888401/file/2022_Ladaigue_et_al_iScience.PDF %L irsn-03888401 %U https://irsn.hal.science/irsn-03888401 %~ CEA %~ IRSN %~ CNRS %~ CEA-UPSAY %~ UNIV-PARIS-SACLAY %~ JACOB %~ CEA-DRF %~ UNIV-LILLE %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ UP-SCIENCES %~ UNIVERSITE-PARIS-SACLAY %~ SU-TI %~ UMR-SGCSR %~ ALLIANCE-SU %~ PSESANTE %~ SERAMED