A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment - IRSN - Institut de radioprotection et de sûreté nucléaire Accéder directement au contenu
Article Dans Une Revue iScience Année : 2022

A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment

Chunxue Han
  • Fonction : Auteur

Résumé

Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions. Trimmed by the class I α-mannosidases, these structures are quite rare in normal conditions. Here, we show that the expression of the endothelial α-mannosidase MAN1C1 protein decreases after irradiation. We modeled two crucial steps in monocyte recruitment by developing in vitro real-time imaging models. Inhibition of MAN1C1 expression by siRNA gene silencing increases the abundance of high-mannose N-glycans, improves the adhesion of monocytes on endothelial cells in flow conditions and, in contrast, decreases radiation-induced transendothelial migration of monocytes. Consistently, overexpression of MAN1C1 in endothelial cells using lentiviral vectors decreases the abundance of high-mannose N-glycans and monocyte adhesion and enhances transendothelial migration of monocytes. Hence, we propose a role for endothelial MAN1C1 in the recruitment of monocytes, particularly in the adhesion step to the endothelium.
Fichier principal
Vignette du fichier
2022_Ladaigue_et_al_iScience.PDF (14.82 Mo) Télécharger le fichier
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

irsn-03888401 , version 1 (07-12-2022)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Ségolène Ladaigue, Anne-Charlotte Lefranc, Khadidiatou Balde, Monica Quitoco, Emilie Bacquer, et al.. A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment. iScience, 2022, 25 (12), pp.105482. ⟨10.1016/j.isci.2022.105482⟩. ⟨irsn-03888401⟩
72 Consultations
160 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More