PyNjoy2016 was born from the fusion of PyNjoy2012 and NJOY2016. This new software is dedicated to the production of libraries for DRAGON5 and SERPENT2, in DRAGLIB and ACE formats, respectively, for deterministic and Monte-Carlo calculations, from a nuclear data evaluation in ENDF-6 format. Among the various improvements over its predecessor, PyNjoy2016 supports the advanced energy deposition model of SERPENT2. It also includes CPU-parallelized features to facilitate the propagation of uncertainties by Total Monte-Carlo method. For that, it relies either on SANDY code, called on-the-fly, or on presampled random TENDL files. Moreover, it allows consistent production of ACE and DRAGLIB files, deriving from the same NJOY computations. The exact deterministic bias of a specific DRAGON5 calculation scheme can therefore be calculated. In contrast, the usual comparisons between deterministic and Monte-Carlo methods are often not based on consistently produced libraries, leading to an error in the calculated deterministic bias. Finally, its fully open source nature (BSD license) ensures a wider accessibility than previous versions of PyNjoy.